anti stat3 fluorescein isothiocyanate  (R&D Systems)

Journal: Oncotarget

Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells

doi: 10.18632/oncotarget.6990

Figure Lengend Snippet: A. The effect of AC-73 on MMP-2 mRNA level in SMMC-7721 cells. MMP-2 mRNA levels were detected using real-time PCR after treatment with DMSO or different concentrations of AC-73. Here, 20 μg/ml HAb18 was used as a positive control. B. Gelatin zymography analysis of MMP-2 secretion. Top, representative image; bottom, quantification of the grayscale analysis in three independent experiments. C, D. CD147, ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells treated with different concentrations of AC-73. E, F. ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells with different treatments. AC-73 was used at a concentration of 10 μM, the ERK1/2 inhibitor PD0325901 was used at 1 μM, and the STAT3 inhibitor WP1066 was used at 10 μM. G, H. ERK1/2 and STAT3 activities were examined in total lysates of HCC and HCC CD147 −/− cells using Western blotting. All the Histograms show quantification of the gray scale analysis for western blotting. The bars represent each sample performed in triplicate and the error bars indicate ± SD. (*** P < 0.001, ** P < 0.01, * P < 0.05, # P > 0.05), one-way ANOVA (H) for multiple comparisons (A, B, D and F), Student's t -test for two comparisons (H).

Article Snippet: Antibodies against STAT3 and p-STAT3 (Ser727) were obtained from Biorbyt LLC (San Francisco, CA).

Techniques: Real-time Polymerase Chain Reaction, Positive Control, Zymography, Expressing, Concentration Assay, Western Blot

Journal: Oncotarget

Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells

doi: 10.18632/oncotarget.6990

Figure Lengend Snippet: A, B. Metastasis assay by intrahepatic injection of SMMC-7721 cells in nude mice. Liver were excised for examination, scale bars: 1cm. Intrahepatic metastases in each group, as determined by calculation of metastatic lesions in the whole liver ( n = 5). C, D. Phosphorylation of ERK1/2 and STAT3 was examined in total lysates of original orthotopic tumors from each group, and histogram shows quantification of the gray scale analysis for western blotting. E. Phosphorylation of ERK1/2 and STAT3, MMP-2 and CD147 expression in original orthotopic tumors at 200× magnification, as determined by IHC staining analysis following treatment with AC-73. Each inset shows images obtained at 400× magnification, scale bars: 100 μm.

Article Snippet: Antibodies against STAT3 and p-STAT3 (Ser727) were obtained from Biorbyt LLC (San Francisco, CA).

Techniques: Injection, Phospho-proteomics, Western Blot, Expressing, Immunohistochemistry

Journal: Oncotarget

Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells

doi: 10.18632/oncotarget.6990

Figure Lengend Snippet: A proposed working model for AC-73-mediated suppression of CD147/ERK1/2/STAT3 signaling to inhibit HCC metastasis

Article Snippet: Antibodies against STAT3 and p-STAT3 (Ser727) were obtained from Biorbyt LLC (San Francisco, CA).

Techniques: