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anti stat3 fluorescein isothiocyanate  (R&D Systems)


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    R&D Systems anti stat3 fluorescein isothiocyanate
    Anti Stat3 Fluorescein Isothiocyanate, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 443 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+stat3+fitc/FITC+Antibody/pmc12396324-307-14-18
    Average 95 stars, based on 443 article reviews
    anti stat3 fluorescein isothiocyanate - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: ZNF341 controls STAT3 expression and thereby immunocompetence
    Article Snippet: Cells were fixed (Lyse/Fix buffer, BD Biosciences) and permeabilized (Fix/Perm III buffer, BD Biosciences) according to the manufacturer ́s instructions. .. Cells were stained with anti-STAT3-FITC (clone #232209; R&D Systems Wiesbaden-Nordenstadt, Germany), anti CD19 BV421 (clone HIB19, Biolegend), or with phospho-specific PE-coupled anti-STAT3 (pY705) antibodies (clone 4/P-STAT3, BD Biosciences), CD3-FITC (clone SK7, BD Bioscience). .. Fixable viability dye eFluor 506 (eBioscience, Frankfurt, Germany) was used according to the manufacturer ́s instructions.

    Article Title: ER stress abrogates the immunosuppressive effect of IL-10 on human macrophages through inhibition of STAT3 activation
    Article Snippet: For intracellular staining cells were stimulated with IL-10 or IL-6 for 15 min and subsequently, fixed with 4% paraformaldehyde (Thermo Scientific) for 15 min at 37 °C washed and permeabilized using ice-cold methanol for at least 60 min at − 20 °C [ ]. .. Staining was done using the following antibodies: anti-pSTAT3(S727)-PE (558557; BD Biosciences), anti-pSTAT3(Y705) (9145S; Cell Signaling), anti-STAT3 (12640S; Cell Signaling), anti-pSTAT3(S727) (94994S; Cell Signaling), and anti-STAT3-FITC (IC1799F; R&D Systems). .. Fluorescence was measured using a FACSCanto II (BD Biosciences).



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    A. The effect of AC-73 on MMP-2 mRNA level in SMMC-7721 cells. MMP-2 mRNA levels were detected using real-time PCR after treatment with DMSO or different concentrations of AC-73. Here, 20 μg/ml HAb18 was used as a positive control. B. Gelatin zymography analysis of MMP-2 secretion. Top, representative image; bottom, quantification of the grayscale analysis in three independent experiments. C, D. CD147, ERK1/2 and <t>STAT3</t> activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells treated with different concentrations of AC-73. E, F. ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells with different treatments. AC-73 was used at a concentration of 10 μM, the ERK1/2 inhibitor PD0325901 was used at 1 μM, and the STAT3 inhibitor WP1066 was used at 10 μM. G, H. ERK1/2 and STAT3 activities were examined in total lysates of HCC and HCC CD147 −/− cells using Western blotting. All the Histograms show quantification of the gray scale analysis for western blotting. The bars represent each sample performed in triplicate and the error bars indicate ± SD. (*** P < 0.001, ** P < 0.01, * P < 0.05, # P > 0.05), one-way ANOVA (H) for multiple comparisons (A, B, D and F), Student's t -test for two comparisons (H).
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    A. The effect of AC-73 on MMP-2 mRNA level in SMMC-7721 cells. MMP-2 mRNA levels were detected using real-time PCR after treatment with DMSO or different concentrations of AC-73. Here, 20 μg/ml HAb18 was used as a positive control. B. Gelatin zymography analysis of MMP-2 secretion. Top, representative image; bottom, quantification of the grayscale analysis in three independent experiments. C, D. CD147, ERK1/2 and <t>STAT3</t> activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells treated with different concentrations of AC-73. E, F. ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells with different treatments. AC-73 was used at a concentration of 10 μM, the ERK1/2 inhibitor PD0325901 was used at 1 μM, and the STAT3 inhibitor WP1066 was used at 10 μM. G, H. ERK1/2 and STAT3 activities were examined in total lysates of HCC and HCC CD147 −/− cells using Western blotting. All the Histograms show quantification of the gray scale analysis for western blotting. The bars represent each sample performed in triplicate and the error bars indicate ± SD. (*** P < 0.001, ** P < 0.01, * P < 0.05, # P > 0.05), one-way ANOVA (H) for multiple comparisons (A, B, D and F), Student's t -test for two comparisons (H).
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    Image Search Results


    A. The effect of AC-73 on MMP-2 mRNA level in SMMC-7721 cells. MMP-2 mRNA levels were detected using real-time PCR after treatment with DMSO or different concentrations of AC-73. Here, 20 μg/ml HAb18 was used as a positive control. B. Gelatin zymography analysis of MMP-2 secretion. Top, representative image; bottom, quantification of the grayscale analysis in three independent experiments. C, D. CD147, ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells treated with different concentrations of AC-73. E, F. ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells with different treatments. AC-73 was used at a concentration of 10 μM, the ERK1/2 inhibitor PD0325901 was used at 1 μM, and the STAT3 inhibitor WP1066 was used at 10 μM. G, H. ERK1/2 and STAT3 activities were examined in total lysates of HCC and HCC CD147 −/− cells using Western blotting. All the Histograms show quantification of the gray scale analysis for western blotting. The bars represent each sample performed in triplicate and the error bars indicate ± SD. (*** P < 0.001, ** P < 0.01, * P < 0.05, # P > 0.05), one-way ANOVA (H) for multiple comparisons (A, B, D and F), Student's t -test for two comparisons (H).

    Journal: Oncotarget

    Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells

    doi: 10.18632/oncotarget.6990

    Figure Lengend Snippet: A. The effect of AC-73 on MMP-2 mRNA level in SMMC-7721 cells. MMP-2 mRNA levels were detected using real-time PCR after treatment with DMSO or different concentrations of AC-73. Here, 20 μg/ml HAb18 was used as a positive control. B. Gelatin zymography analysis of MMP-2 secretion. Top, representative image; bottom, quantification of the grayscale analysis in three independent experiments. C, D. CD147, ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells treated with different concentrations of AC-73. E, F. ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells with different treatments. AC-73 was used at a concentration of 10 μM, the ERK1/2 inhibitor PD0325901 was used at 1 μM, and the STAT3 inhibitor WP1066 was used at 10 μM. G, H. ERK1/2 and STAT3 activities were examined in total lysates of HCC and HCC CD147 −/− cells using Western blotting. All the Histograms show quantification of the gray scale analysis for western blotting. The bars represent each sample performed in triplicate and the error bars indicate ± SD. (*** P < 0.001, ** P < 0.01, * P < 0.05, # P > 0.05), one-way ANOVA (H) for multiple comparisons (A, B, D and F), Student's t -test for two comparisons (H).

    Article Snippet: Antibodies against STAT3 and p-STAT3 (Ser727) were obtained from Biorbyt LLC (San Francisco, CA).

    Techniques: Real-time Polymerase Chain Reaction, Positive Control, Zymography, Expressing, Concentration Assay, Western Blot

    A, B. Metastasis assay by intrahepatic injection of SMMC-7721 cells in nude mice. Liver were excised for examination, scale bars: 1cm. Intrahepatic metastases in each group, as determined by calculation of metastatic lesions in the whole liver ( n = 5). C, D. Phosphorylation of ERK1/2 and STAT3 was examined in total lysates of original orthotopic tumors from each group, and histogram shows quantification of the gray scale analysis for western blotting. E. Phosphorylation of ERK1/2 and STAT3, MMP-2 and CD147 expression in original orthotopic tumors at 200× magnification, as determined by IHC staining analysis following treatment with AC-73. Each inset shows images obtained at 400× magnification, scale bars: 100 μm.

    Journal: Oncotarget

    Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells

    doi: 10.18632/oncotarget.6990

    Figure Lengend Snippet: A, B. Metastasis assay by intrahepatic injection of SMMC-7721 cells in nude mice. Liver were excised for examination, scale bars: 1cm. Intrahepatic metastases in each group, as determined by calculation of metastatic lesions in the whole liver ( n = 5). C, D. Phosphorylation of ERK1/2 and STAT3 was examined in total lysates of original orthotopic tumors from each group, and histogram shows quantification of the gray scale analysis for western blotting. E. Phosphorylation of ERK1/2 and STAT3, MMP-2 and CD147 expression in original orthotopic tumors at 200× magnification, as determined by IHC staining analysis following treatment with AC-73. Each inset shows images obtained at 400× magnification, scale bars: 100 μm.

    Article Snippet: Antibodies against STAT3 and p-STAT3 (Ser727) were obtained from Biorbyt LLC (San Francisco, CA).

    Techniques: Injection, Phospho-proteomics, Western Blot, Expressing, Immunohistochemistry

    A proposed working model for AC-73-mediated suppression of CD147/ERK1/2/STAT3 signaling to inhibit HCC metastasis

    Journal: Oncotarget

    Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells

    doi: 10.18632/oncotarget.6990

    Figure Lengend Snippet: A proposed working model for AC-73-mediated suppression of CD147/ERK1/2/STAT3 signaling to inhibit HCC metastasis

    Article Snippet: Antibodies against STAT3 and p-STAT3 (Ser727) were obtained from Biorbyt LLC (San Francisco, CA).

    Techniques:

    A. The effect of AC-73 on MMP-2 mRNA level in SMMC-7721 cells. MMP-2 mRNA levels were detected using real-time PCR after treatment with DMSO or different concentrations of AC-73. Here, 20 μg/ml HAb18 was used as a positive control. B. Gelatin zymography analysis of MMP-2 secretion. Top, representative image; bottom, quantification of the grayscale analysis in three independent experiments. C, D. CD147, ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells treated with different concentrations of AC-73. E, F. ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells with different treatments. AC-73 was used at a concentration of 10 μM, the ERK1/2 inhibitor PD0325901 was used at 1 μM, and the STAT3 inhibitor WP1066 was used at 10 μM. G, H. ERK1/2 and STAT3 activities were examined in total lysates of HCC and HCC CD147 −/− cells using Western blotting. All the Histograms show quantification of the gray scale analysis for western blotting. The bars represent each sample performed in triplicate and the error bars indicate ± SD. (*** P < 0.001, ** P < 0.01, * P < 0.05, # P > 0.05), one-way ANOVA (H) for multiple comparisons (A, B, D and F), Student's t -test for two comparisons (H).

    Journal: Oncotarget

    Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells

    doi: 10.18632/oncotarget.6990

    Figure Lengend Snippet: A. The effect of AC-73 on MMP-2 mRNA level in SMMC-7721 cells. MMP-2 mRNA levels were detected using real-time PCR after treatment with DMSO or different concentrations of AC-73. Here, 20 μg/ml HAb18 was used as a positive control. B. Gelatin zymography analysis of MMP-2 secretion. Top, representative image; bottom, quantification of the grayscale analysis in three independent experiments. C, D. CD147, ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells treated with different concentrations of AC-73. E, F. ERK1/2 and STAT3 activities and total ERK1/2 and STAT3 expression were examined in SMMC-7721 cells with different treatments. AC-73 was used at a concentration of 10 μM, the ERK1/2 inhibitor PD0325901 was used at 1 μM, and the STAT3 inhibitor WP1066 was used at 10 μM. G, H. ERK1/2 and STAT3 activities were examined in total lysates of HCC and HCC CD147 −/− cells using Western blotting. All the Histograms show quantification of the gray scale analysis for western blotting. The bars represent each sample performed in triplicate and the error bars indicate ± SD. (*** P < 0.001, ** P < 0.01, * P < 0.05, # P > 0.05), one-way ANOVA (H) for multiple comparisons (A, B, D and F), Student's t -test for two comparisons (H).

    Article Snippet: Antibodies against STAT3 and p-STAT3 (Ser727) were obtained from Biorbyt LLC (San Francisco, CA).

    Techniques: Real-time Polymerase Chain Reaction, Positive Control, Zymography, Expressing, Concentration Assay, Western Blot

    A, B. Metastasis assay by intrahepatic injection of SMMC-7721 cells in nude mice. Liver were excised for examination, scale bars: 1cm. Intrahepatic metastases in each group, as determined by calculation of metastatic lesions in the whole liver ( n = 5). C, D. Phosphorylation of ERK1/2 and STAT3 was examined in total lysates of original orthotopic tumors from each group, and histogram shows quantification of the gray scale analysis for western blotting. E. Phosphorylation of ERK1/2 and STAT3, MMP-2 and CD147 expression in original orthotopic tumors at 200× magnification, as determined by IHC staining analysis following treatment with AC-73. Each inset shows images obtained at 400× magnification, scale bars: 100 μm.

    Journal: Oncotarget

    Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells

    doi: 10.18632/oncotarget.6990

    Figure Lengend Snippet: A, B. Metastasis assay by intrahepatic injection of SMMC-7721 cells in nude mice. Liver were excised for examination, scale bars: 1cm. Intrahepatic metastases in each group, as determined by calculation of metastatic lesions in the whole liver ( n = 5). C, D. Phosphorylation of ERK1/2 and STAT3 was examined in total lysates of original orthotopic tumors from each group, and histogram shows quantification of the gray scale analysis for western blotting. E. Phosphorylation of ERK1/2 and STAT3, MMP-2 and CD147 expression in original orthotopic tumors at 200× magnification, as determined by IHC staining analysis following treatment with AC-73. Each inset shows images obtained at 400× magnification, scale bars: 100 μm.

    Article Snippet: Antibodies against STAT3 and p-STAT3 (Ser727) were obtained from Biorbyt LLC (San Francisco, CA).

    Techniques: Injection, Phospho-proteomics, Western Blot, Expressing, Immunohistochemistry

    A proposed working model for AC-73-mediated suppression of CD147/ERK1/2/STAT3 signaling to inhibit HCC metastasis

    Journal: Oncotarget

    Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells

    doi: 10.18632/oncotarget.6990

    Figure Lengend Snippet: A proposed working model for AC-73-mediated suppression of CD147/ERK1/2/STAT3 signaling to inhibit HCC metastasis

    Article Snippet: Antibodies against STAT3 and p-STAT3 (Ser727) were obtained from Biorbyt LLC (San Francisco, CA).

    Techniques: